Antibody-membrane switch (AMS) technology for facile cell line development
نویسندگان
چکیده
منابع مشابه
Antibody-membrane switch (AMS) technology for facile cell line development.
Generation of high-productivity cell lines remains a major bottleneck in therapeutic antibody development. Conventional cell line development often depends on gene amplification methodologies using dihydrofolate reductase or glutamine synthetase. Higher productivity is associated with an increased gene copy number. However, lack of selection pressure under the conditions of large-scale manufact...
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Background Chinese hamster ovary (CHO) cells are the most widely used host for large scale production of recombinant therapeutic proteins. A combination of several gene editing approaches applied to Novartis proprietary CHO cell line resulted in a superior cell line with a significant increase of titer and improved product quality. Inter alia we have surprisingly identified a key protease respo...
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conclusions switching ams off may increase biventricular pacing in some patients. case presentation in this case series, we present a combination of far field sensing and automatic mode switching (ams) in six patients. it is found that this combination causes ventricular sensing (vs) episodes with wide qrs and no synchronization. we turn off the ams and alleviate the problem. introduction cardi...
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INTRODUCTION Cardiac resynchronization devices are part of modern heart failure management. After implantation, we analyze and program devices in an attempt to ensure their success. Biventricular pacing should be 98% or more for the lowest mortality and best symptom improvement. CASE PRESENTATION In this case series, we present a combination of far field sensing and automatic mode switching (...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2014
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzu039